ABSTRACT
Objective: To investigate the effect of autoinflation on the prognosis of OME in children. Methods: Total of 325 pediatric patients, age ranged from 3 to 8 years, with OME(486 ears)diagnosed in our department from January 2019 to January 2020 were collected. Among them, 177 were males, 148 were females. Disease course ranged from 25 to 86 days. According to watchful waiting and autoinflation application during follow-up, these children were divided into two groups including 183 cases(271 ears) and 142 cases(215 ears), respectively. The average auditory threshold and tympanogram curve type in two groups were analyzed in the period of 3 months follow-up, and the recovery of OME was evaluated. Results: At the end of 1- and 2- month follow-up, the auditory threshold of patients in autoinflation group was significantly lower than that in watchful waiting group (t=2.139 5 and 2.680 6, P<0.05). However, at the end of 3- month follow-up, there was no significant difference between two groups (t=1.158 5, P>0.05). At the end of 1-, 2- and 3- month follow-up, 89 (33%, 89/271), 200 (74%, 200/271), 220 (81%, 220/271) and 176 (82%, 176/215), 178 (83%, 178/215), 183 (85%, 183/215) ears in watchful waiting group and autoinflation group had a hearing threshold <20 dB HL, respectively, in which ears with auditory threshold<20 dB HL in watchful waiting group were significantly less than those in autoinflation group at the end of 1 and 2 month follow-up (P<0.05), However, ears with auditory threshold<20 dB HL in watchful waiting group were not significantly different from that in the autoflation group at the end of 3- month follow-up (P>0.05). The proportion of ears with type A tympanogram curve was 74%(159/215), 79%(170/215), and 85%(183/215) at the end of 1-, 2- and 3- month follow-up in autoinflation group and 36%(98/271), 71%(192/271) and 76%(206/271) in watchful waiting group, respectively. Proportion of ears with type A tympanogram curve in autoflation group was significantly higher than that in watchful waiting group (P<0.05). Conclusion: Autoinflation can improve the hearing of children with OME in early stage, restore normal middle ear pressure, increase recovery rate, and reduce the choice of surgical treatment of OME.
Subject(s)
Child , Child, Preschool , Female , Humans , Male , Auditory Threshold , Hearing , Middle Ear Ventilation , Otitis Media with Effusion/diagnosis , PrognosisABSTRACT
Objective As the photostability of calcium ions (Ca2+) indicators is an important property for indicating the temporal features of cytosolic Ca2+ in cells, this study aims to quantitatively measure the light-induced fluorescence enhancement in cells stained with Ca2+ indicators. Methods Five cell lines, MC3T3-E1, RAW264.7, MLO-Y4, MEF3T3 and HEK293, were exposed to the light with five levels of optical power, respectively, so as to investigate the light induced responses of two commonly-used Ca2+ indicators, Fluo-4 AM and Oregon green. The light-induced fluorescence enhancement, the succeeding photobleaching and the thapsigargin (TG)-induced responsive peak followed by were observed. The characteristic parameters of responsive peaks were further analyzed. Results Light with higher power level would induce the fluorescence enhancement for both Fluo-4 AM or Oregon green, while the responsive percentage as well as the magnitude and time span of light-induced peak of Oregon green-stained cells were significantly lower than those of Fluo-4 AM-stained cells. Conclusions The use of Oregon green with low power level light shows better photostability to indicate the intracellular Ca2+.